rabbit antibody against psbe Search Results


rabbit antibodies against d2, psbr psbe  (Agrisera)
90
Agrisera rabbit antibodies against d2, psbr psbe
Cross-linking of PsbP with PSII membranes using EDC and sulfo-NHS. NaCl-washed PSII membranes were incubated with 6.25 mm EDC and 5 mm sulfo-NHS in either (A) the presence or (B) the absence of WT PsbP. Cross-linked proteins corresponding to 2 μg of Chl were loaded onto each lane. The proteins were immunodetected with antisera against CP47, CP43, <t>PsbO,</t> <t>D2,</t> D1, PsbP, PsbR, and <t>PsbE,</t> as shown on each lane. Protein size markers are shown on the left. C, NaCl-washed PSII was treated with 6.25 mm EDC and 5 mm sulfo-NHS to activate the carboxyl groups on the PSII. After activation, the PSII was washed and incubated either in the presence (+PsbP) or in the absence (−PsbP) of PsbP. As a positive control, the sample used in A was analyzed simultaneously.
Rabbit Antibodies Against D2, Psbr Psbe, supplied by Agrisera, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit antibodies against d2, psbr psbe - by Bioz Stars, 2026-04
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Cross-linking of PsbP with PSII membranes using EDC and sulfo-NHS. NaCl-washed PSII membranes were incubated with 6.25 mm EDC and 5 mm sulfo-NHS in either (A) the presence or (B) the absence of WT PsbP. Cross-linked proteins corresponding to 2 μg of Chl were loaded onto each lane. The proteins were immunodetected with antisera against CP47, CP43, PsbO, D2, D1, PsbP, PsbR, and PsbE, as shown on each lane. Protein size markers are shown on the left. C, NaCl-washed PSII was treated with 6.25 mm EDC and 5 mm sulfo-NHS to activate the carboxyl groups on the PSII. After activation, the PSII was washed and incubated either in the presence (+PsbP) or in the absence (−PsbP) of PsbP. As a positive control, the sample used in A was analyzed simultaneously.

Journal: The Journal of Biological Chemistry

Article Title: The Conserved His-144 in the PsbP Protein Is Important for the Interaction between the PsbP N-terminus and the Cyt b 559 Subunit of Photosystem II *

doi: 10.1074/jbc.M112.385286

Figure Lengend Snippet: Cross-linking of PsbP with PSII membranes using EDC and sulfo-NHS. NaCl-washed PSII membranes were incubated with 6.25 mm EDC and 5 mm sulfo-NHS in either (A) the presence or (B) the absence of WT PsbP. Cross-linked proteins corresponding to 2 μg of Chl were loaded onto each lane. The proteins were immunodetected with antisera against CP47, CP43, PsbO, D2, D1, PsbP, PsbR, and PsbE, as shown on each lane. Protein size markers are shown on the left. C, NaCl-washed PSII was treated with 6.25 mm EDC and 5 mm sulfo-NHS to activate the carboxyl groups on the PSII. After activation, the PSII was washed and incubated either in the presence (+PsbP) or in the absence (−PsbP) of PsbP. As a positive control, the sample used in A was analyzed simultaneously.

Article Snippet: Rabbit antibodies against D2, PsbR and PsbE were purchased from Agrisera.

Techniques: Incubation, Activation Assay, Positive Control